34
          
        
        
          
            Tecnología y Ciencias del Agua
          
        
        
          , vol. VIII, núm. 3, mayo-junio de 2017, pp. 27-37
        
        
          Aburto-Medina
        
        
          
            et al
          
        
        
          .
        
        
          
            , Prevalence of
          
        
        
          Enterobacteriaceae
        
        
          
            and contaminants survey in sediments of the Atoyac River
          
        
        
          •
        
        
          ISSN 2007-2422
        
        
          Thus, there may be health risks since these wa-
        
        
          ters are later used to irrigate several crops such
        
        
          as maize, sorghum, and alfalfa in lands above
        
        
          the dam (Larenas-Bazán, 2010). Therefore, it
        
        
          is of great importance to identify the effluents
        
        
          located after the wastewater treatment process
        
        
          and/or review the water quality coming out of
        
        
          those treatment facilities, another aid would be
        
        
          the use of microbial source tracking markers
        
        
          such as the stanols (Derrien
        
        
          
            et al
          
        
        
          ., 2012) in order
        
        
          to identify faecal contamination sources. It is
        
        
          equally important to look for catabolic and
        
        
          pathogenicity genes in the environmental
        
        
          samples in order to properly assess the mi-
        
        
          crobial diversity degrading potential and its
        
        
          pathogenicity.
        
        
          Figure 2. Phylogenetic tree of the
        
        
          
            Enterobacteriales
          
        
        
          order based on partial 16SrDNA sequences showing the relationships among
        
        
          bacterial clone sequences from this study and members of the
        
        
          
            Enterobacteriaceae
          
        
        
          family found in the database. Jukes and Cantor
        
        
          distance, neighbour-joining method, outgroup:
        
        
          
            Comamonas denitrifications
          
        
        
          . Clones from this study are in bold, A: Atoyac.
        
        
          A number in parenthesis indicates the number of clones obtained for each sequence. Accession numbers of the sequences
        
        
          retrieved from the databases are also indicated in parenthesis. Bootstrap values above 50% are shown at branch nodes (per 1000
        
        
          trials). 16S rRNA sequence similarity over the region 800 to 1390 based on
        
        
          
            E. coli
          
        
        
          numbering.